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HSP90AB1 Antibody

HSP90AB1 Antibody

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CSB-PA00109A0Rb

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Product Code: CSB-PA00109A0Rb
Size: 50μg/100μg
Host Species: Rabbit
Species reactivity: Human|Mouse|Rat|Zebrafish
Test application: ELISA, WB;Recommended dilution:WB:1:1000-1:5000

More Information
Aliases	Heat shock protein HSP 90-beta (HSP 90) (Heat shock 84 kDa) (HSP 84) (HSP84), HSP90AB1, HSP90B HSPC2 HSPCB
Alternatives to SCBT	sc-1057
Target/Protein	HSP90AB1
Uniprot NO.	P08238
Immunogen	Recombinant Human human Heat shock protein HSP 90-beta protein (150-355AA)
Immunogen Species	Human
Host species	Rabbit
Species Reactivity	Human, Mouse, Rat, Zebrafish
Clonality	Polyclonal
Isotype	IgG
Modification	unmodified
Applications	WB, IHC, IF, ELISA
Background	Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Conjugates	Non-conjugated
Form	Liquid
Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Research areas	Signal Transduction

HSP90AB1 Antibody Images

Western blot
All lanes: Hsp90ab1 antibody at 2μg/ml
Lane 1: Mouse lung tissue
Lane 2: Rat lung tissue
Lane 3: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83

Western Blot
Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate
All lanes: HSP90AB1 antibody at 2μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa

Western Blot
Positive WB detected in: HepG2 cell
All lanes: CHD4 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa

IHC image of CSB-PA00109A0Rb diluted at 1:200 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA00109A0Rb at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).