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CTNNB1 Antibody

CTNNB1 Antibody

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CSB-PA00174A0Rb

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Product Code: CSB-PA00174A0Rb
Size: 50μg/100μg
Host Species: Rabbit
Species reactivity: Human|Mouse|Zebrafish
Test application: ELISA, WB, IHC;Recommended dilution:WB:1:1000-1:5000, IHC:1:20-1:200

More Information
Aliases	Catenin beta-1 (Beta-catenin), CTNNB1, CTNNB
Alternatives to SCBT	sc-31000/sc-31001/sc-7199
Target/Protein	CTNNB1
Uniprot NO.	P35222
Immunogen	Recombinant Human Catenin beta-1 protein (2-781AA)
Immunogen Species	Human
Host species	Rabbit
Species Reactivity	Human
Clonality	Polyclonal
Isotype	IgG
Modification	unmodified
Applications	WB, IHC, IF, ELISA, IP
Background	Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Involved in signal transduction through the Wnt pathway. Nuclear beta-catenin it involved in transcriptional regulation by association with transcription factors of the TCF/LEF family.
Conjugates	Non-conjugated
Form	Liquid
Buffer	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Research areas	Signal Transduction

CTNNB1 Antibody Images

Immunofluorescence staining of HepG2 cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating CTNNB1 in HT29 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA00174A0Rb in HT29 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA00174A0Rb (8μg) + HT29 whole cell lysate (500μg)
Lane 3: HT29 whole cell lysate (20μg)

Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA00174A0Rb at dilution 1:100

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate
All lanes: CTNNB1 antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00174A0Rb at dilution of 1:100

IHC image of CSB-PA00174A0Rb diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).