CTNNB1 Antibody
As low as
$166.00
In stock
Product Code
CSB-PA00174A0Rb
Product Code: CSB-PA00174A0Rb
Size: 50μg/100μg
Host Species: Rabbit
Species reactivity: Human|Mouse|Zebrafish
Test application: ELISA, WB, IHC;Recommended dilution:WB:1:1000-1:5000, IHC:1:20-1:200
Size: 50μg/100μg
Host Species: Rabbit
Species reactivity: Human|Mouse|Zebrafish
Test application: ELISA, WB, IHC;Recommended dilution:WB:1:1000-1:5000, IHC:1:20-1:200
Aliases | Catenin beta-1 (Beta-catenin), CTNNB1, CTNNB |
---|---|
Alternatives to SCBT | sc-31000/sc-31001/sc-7199 |
Target/Protein | CTNNB1 |
Uniprot NO. | P35222 |
Immunogen | Recombinant Human Catenin beta-1 protein (2-781AA) |
Immunogen Species | Human |
Host species | Rabbit |
Species Reactivity | Human |
Clonality | Polyclonal |
Isotype | IgG |
Modification | unmodified |
Applications | WB, IHC, IF, ELISA, IP |
Background | Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. Involved in signal transduction through the Wnt pathway. Nuclear beta-catenin it involved in transcriptional regulation by association with transcription factors of the TCF/LEF family. |
Conjugates | Non-conjugated |
Form | Liquid |
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Research areas | Signal Transduction |
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CTNNB1 Antibody Images

Immunofluorescence staining of HepG2 cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunoprecipitating CTNNB1 in HT29 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA00174A0Rb in HT29 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA00174A0Rb (8μg) + HT29 whole cell lysate (500μg)
Lane 3: HT29 whole cell lysate (20μg)
Lane 1: Rabbit control IgG instead of CSB-PA00174A0Rb in HT29 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
Lane 2: CSB-PA00174A0Rb (8μg) + HT29 whole cell lysate (500μg)
Lane 3: HT29 whole cell lysate (20μg)

Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA00174A0Rb at dilution 1:100

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate
All lanes: CTNNB1 antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
Positive WB detected in: SH-SY5Y whole cell lysate, Hela whole cell lysate
All lanes: CTNNB1 antibody at 2.5μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA00174A0Rb at dilution of 1:100

IHC image of CSB-PA00174A0Rb diluted at 1:500 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA00174A0Rb at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).