NES Monoclonal Antibody
$210.00
In stock
Product Code
CSB-MA0157131A0m
Product Code:CSB-MA0157131A0m
Size:50μg/100μg
Host Species:Mouse
Species reactivity:Human
Test application:ELISA, WB, IHC, IF, FC;Recommended dilution:WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Size:50μg/100μg
Host Species:Mouse
Species reactivity:Human
Test application:ELISA, WB, IHC, IF, FC;Recommended dilution:WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
| Aliases | NES, Nbla00170, Nestin |
|---|---|
| Alternatives to SCBT | sc-23927/sc-71665/sc-73489 |
| Target/Protein | NES |
| Uniprot NO. | P48681 |
| Immunogen | Recombinant nestin Protein |
| Immunogen Species | Human |
| Host species | Mouse |
| Species Reactivity | Human |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Modification | unmodified |
| Applications | WB, IHC, IF, ELISA, FC |
| Background | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. |
| Conjugates | Non-conjugated |
| Form | liquid |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Research areas | Neuroscience |
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NES Monoclonal Antibody Images
Western Blot
Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate
All lanes: NES antibody at 3 ug/ml
Predicted band size: 260 kDa
Observed band size: 260 kDa
Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate
All lanes: NES antibody at 3 ug/ml
Predicted band size: 260 kDa
Observed band size: 260 kDa
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-MA0157131A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA0157131A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human melanoma using CSB-MA0157131A0m at dilution of 1:100.
Immunofluorescent analysis of Hela cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of PC-3 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of U251 cells using CSB-MA0157131A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing U251 cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Overlay histogram showing Hela cells stained with CSB-MA0157131A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x106cells) used under the same conditions. Acquisition of >10, 000 events was performed.