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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Research areas
Neuroscience
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GFAP monoclonal antibody Images
Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: GFAP antibody at 2.7ug/ml
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100.
Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing SY5Y cells stained with CSB-MA009369A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block
