GFAP monoclonal antibody
$210.00
In stock
Product Code
CSB-MA009369A0m
Product Code:CSB-MA009369A0m
Size:50μg/100μg
Host Species:Mouse
Species reactivity:Human|Mouse|Rat
Test application:ELISA, WB, IHC, IF, FC;Recommended dilution:WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Size:50μg/100μg
Host Species:Mouse
Species reactivity:Human|Mouse|Rat
Test application:ELISA, WB, IHC, IF, FC;Recommended dilution:WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
| Aliases | Glial fibrillary acidic protein, GFAP |
|---|---|
| Alternatives to SCBT | N/A |
| Target/Protein | GFAP |
| Uniprot NO. | P14136 |
| Immunogen | Recombinant Human GFAP protein (292-432AA) |
| Immunogen Species | Human |
| Host species | Mouse |
| Species Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Modification | unmodified |
| Applications | WB, IHC, IF, ELISA, FC |
| Background | GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Conjugates | Non-conjugated |
| Form | liquid |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Research areas | Neuroscience |
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GFAP monoclonal antibody Images
Western Blot
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: GFAP antibody at 2.7ug/ml
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
Positive WB detected in: Mouse brain tissue, Rat brain tissue
All lanes: GFAP antibody at 2.7ug/ml
Predicted band size: 50, 51 kDa
Observed band size: 50 kDa
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-MA009369A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human glioma using CSB-MA009369A0m at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-MA009369A0m at dilution of 1:100.
Immunofluorescent analysis of A549 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of SY5Y cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of U251 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of U87 cells using CSB-MA009369A0m at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Overlay histogram showing SY5Y cells stained with CSB-MA009369A0m (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG2b (10ug/1x106cells) used under the same conditions. Acquisition of >10, 000 events was performed.