Flag Tag Monoclonal Antibody

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Product Code:CSB-MA000021M0m
Host Species:Mouse
Test application:ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000
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Aliases 3X Flag, DDK tag, DYKDDDDK, Flag, Flag Tag
Immunogen DYKDDDDK(FLAG) synthetic peptide conjugate to KLH
Host species Mouse
Clonality Monoclonal
Isotype IgG2b
Applications WB, IF, ELISA, IP
Background FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
Conjugates Non-conjugated
Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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