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6*His Monoclonal Antibody

As low as $120.00
In stock
Product Code
Product Code:CSB-MA000011M0m
Host Species:Mouse
Test application:ELISA,WB;Recommended dilution:WB:1:500-1:5000
More Information
Aliases 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag
Immunogen HHHHHH(6xHis) synthetic peptide conjugate to KLH
Host species Mouse
Clonality Monoclonal
Isotype IgG2a
Applications WB, ELISA
Background A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound.
Conjugates Non-conjugated
Buffer Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

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