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Aliases | 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag |
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Immunogen | HHHHHH(6xHis) synthetic peptide conjugate to KLH |
Host species | Mouse |
Clonality | Monoclonal |
Isotype | IgG2a |
Applications | WB, ELISA |
Background | A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. |
Conjugates | Non-conjugated |
Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |