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6*His Monoclonal Antibody
As low as
$120.00
In stock
Product Code
CSB-MA000011M0m
Product Code:CSB-MA000011M0m
Size:50μg/100μg
Host Species:Mouse
Test application:ELISA,WB;Recommended dilution:WB:1:500-1:5000
Size:50μg/100μg
Host Species:Mouse
Test application:ELISA,WB;Recommended dilution:WB:1:500-1:5000
| Aliases | 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag |
|---|---|
| Immunogen | HHHHHH(6xHis) synthetic peptide conjugate to KLH |
| Host species | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB, ELISA |
| Background | A HIS tag is a tag of 6 histidines added to the end of the protein that the DNA codes for, so the DNA with contain 6 repeats of the HIS codon. What it is used for is purifying the protein that the DNA codes for. Histidines are positively charged amino acids and a group of 6 of them allows the protein to bind to a negatively charged column. Histidines are also special in that they have a pKa around 7, meaning that once bound to the column they can easily be unbound. |
| Conjugates | Non-conjugated |
| Buffer | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
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6*His Monoclonal Antibody Images
All lanes :Mouse Anti--6*his monoclonal antibody at 1ug/ml
Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
Recombinant proteins per well onto a 15% Tris-HCL polyacr
Western blot analysis of 6*his Epitope Tag was performde by loading various amounts of lysates containing different HIS tagged
Recombinant proteins per well onto a 15% Tris-HCL polyacr
All lanes : Recombinant DST Protein at 30ng/ml
Line 1:Mouse Anti-his monoclonal antibody at 1:64000
Line2:Mouse Anti-his monoclonal antibody at 1:32000
Line3:Mouse Anti-his monoclonal antibody at 1:16000
Line4:Mouse Anti-his monocl
Line 1:Mouse Anti-his monoclonal antibody at 1:64000
Line2:Mouse Anti-his monoclonal antibody at 1:32000
Line3:Mouse Anti-his monoclonal antibody at 1:16000
Line4:Mouse Anti-his monocl